Cetylmyristoleate: the natural way to relieve arthritis pain

A Randomized Clinical Trial
Published 1997,Second Quarter
Dr. H. Siemandi, M.D., et al

Recent published reports offer anecdotal evidence that Cetyl Myristoleate may provide significant amelioration of various arthritic conditions. We set out to perform controlled studies to determine if this material was efficacious, either in the short term, or in some measurable manner, over a much longer period.

A prospective, randomized study design was used to allocate patients to receive Cetyl Myristoleate, Cetyl Myristoleate plus Glucosamine hydrochloride (GH), sea cucumber (SC) and hydrolyzed cartilage (HC) and a placebo.Results. At the start of this study, the duration, severity, and pattern of arthritic episodes were found to be similar in the 3 treatment groups. At the end of the study it was found that the number of arthritic episodes was significantly reduced, and the duration of episode-free time was significantly prolonged, in the two Cetyl Myristoleate groups compared with the placebo group.

Cetyl Myristoleate treatment and Cetyl Myristoleate plus GH, SC & HC were demonstrated to offer significant benefits over the placebo in the prevention of arthritic episodes. It was further determined that these results could not be obtained with other standard arthritic therapies based upon exhaustive reviews of patient records prior to opening of the study. Cetyl Myristoleate and Cetyl Myristoleate plus GH, SC & HC treatment also seems to permit some relief to autoimmune inflammatory diseases, which may prove to be long-term. This finding could provide additional evidence for the theory, reflected by the earlier anecdotal evidence as well as some animal studies, that Cetyl Myristoleate and Cetyl Myristoleate plus GS, SC & HC may prove to be of major benefit in the future treatment of autoimmune diseases.

The terms ARTHRITIS AND PSORIASIS have come to some permanent and some transient. Each condition, however, is typified by certain common elements such as some sort of inflammatory response with resulting pain, various forms of cellular degeneration and frequently, permanent loss of mobility and quality of life.

With the exception of Osteoarthritis, most researchers are beginning to believe all arthritic conditions may have a common, albeit many-faceted, etiology - autoimmune dysfunction. Unfortunately the great number and complexity of immune system components and their diverse interplay has made this theory difficult to prove.

While it has not been proven, the original research done on Cetyl Myristoleate at NIH indicates a direct connection between the observed effect of Cetyl Myristoleate and some ability of Cetyl Myristoleate to correct certain immune dysfunction, which may cause many arthritic conditions.

Patients and Methods

Study design
The study was a 32 week (8 week cycle, 4 in-hospital & 4 in follow-up), multi-centric, double-blind, randomized, placebo-controlled parallel trial that compared the efficacy of Cetyl Myristoleate alone, and Cetyl Myristoleate plus GS, SC & HC, administered over a period of 30 days, with placebo, for the treatment of various forms of autoimmune diseases commonly characterized as arthritis and psoriasis. Out of a dose of 90 grams of total fatty acid esters, 18 grams constituted Cetyl Myristoleate. Those study patients who received the support nutrients GS, SC, & HC were given a total dosage of 18 grams each of these nutrients.

The study was conducted under the auspices of the Joint European Hospital Studies Program. This study was designed by a committee, which consisted of rheumatologists and biostatisticians experienced in the development and execution of clinical trials. Oversight of the study was accomplished by an executive committee, composed of the primary researcher and primary statistician, selected participating investigators, consultants; and an independent sight committee consisting of two experienced federally controlled, state health department rheumatologists and one state health department biostatistician.

Eligibility criteria
Patients were required to have inflammatory arthritis of at least one year duration in at least one peripheral joint, excluding the shoulders and hips. Included in this parameter, affected joints must have had joint tenderness and joint swelling of 2 on a four point scale and joint patient-physician overall assessment of involvement ranging from; none - mild - moderate - severe - very severe.

The patients inducted into this trial for the purposes of psoriatic testing were chosen on generally the same criteria - involvement of epidermal involvement from: none - mild - moderate - severe - very severe. Criteria for exclusion included unwillingness to stop the use of tobacco and caffeinated beverages, at least for the duration of the trial. Tobacco and caffeine use has been reported to greatly hamper the positive (if anecdotal) result of the use of Cetyl Myristoleate. It also should be noted here that the use of any other medication in all forms of arthritis as well as psoriasis were not excluded as it was determined this would limit participation. It was also deemed advisable to approximate as much as possible, conditions that would be found in the average arthritic. One exception to this condition was the exclusion of patients showing sensitivity to salicylates or ibuprofen, which were used as excipients in the placebo.

Potential participants with other severe chronic conditions were excluded, as it was the opinion of the primary investigator that this type of participant would limit the potential successful completion of the study period. All patients had failed to respond to therapy with therapeutic doses of one of the NSAIDs. All patients who took NSAIDs during the trial were required to be on stable dosages for one month prior to entry and throughout the trial. No systemic or intrarticular steroids were used. All patients were fully informed and voluntarily consented to participate in the research program. The study protocol was reviewed and approved by the federally controlled state oversight committee. Prior to entry into this trial, each potential study participant was informed of the nature, duration, and purpose of the study to be administered, and all the potential benefits, inconveniences, and hazards that could reasonably be expected.

Study medication
Patients received either one-half liter of pleasantly flavored oral liquid containing 18 grams of Cetyl Myristoleate or one-half liter of the same liquid with Cetyl Myristoleate. Both liquids were carefully compounded so as not to be able to be differentiated. Each patient was also given 180 capsules of the adjunctive medication containing a total each of 18 grams GL, SC and HC. Identical capsules containing the placebo compound were also distributed. The Cetyl Myristoleate topical liquid was distributed as a 25% concentration in 60-ccs. lightly scented lotion and an identical placebo lotion with Cetyl Myristoleate. The oral liquid was used with meals in one-teaspoon quantities, three times daily. Two capsules of GL, HC & SC were taken with each meal, three times daily. The topical lotion was used as needed and determined by each patient according to his or her own perceived requirement.

Clinical assessment
Outcome measures of disease activity and therapeutic efficacy were obtained at the time of screening (not more than four weeks before study entry), randomization at week zero, and thereafter at weeks; 1, 2, 3, and 4. Outcome measures included a variety of patient-reported, clinical, laboratory and radiographic assessments. Patient self-assessment measures included morning stiffness, night pain, patient overall assessment and Mobility Functional Index as determined by this published procedure. Clinical assessment measures included joint counts, dactylitis, Enthesopathy Index, Spondylitis Articular Index, chest expansion, modified Schober’s test, and finger-to-floor test as detailed elsewhere in this paper. Additionally, the presence of symptomatic keratoderma, phalangeal and digital deformation as measured from a normal range of vertical protrusion at rest were measured. These tests, singularly and collectively were then compiled into a patient-by-patient qualitative scale as; none = O, mild = 1, moderate = 2, severe = 3 and very severe =4. Laboratory assessment. Laboratory evaluation included a urinalysis and complete blood cell count, with leukocyte differential and reticulocyte count. Chemical surveys and a Westergren erythrocyte sedimentation rate (ESR) determination were done at every visit by secondary researchers daily in the two hospital settings. The C-reactive protein (CRP) level was evaluated at the first and last day of the hospital stay. At the screening times, blood was drawn for HLA-B27 typing and RF and ANA determinations.

Radiology assessment
At the screening visit, all patients had the following radiographs performed: anteroposterior views of the pelvis and oblique views of the sacroiliac joints. Adverse drug reactions (ADR’s). Patients were screened for ADR’s at every secondary researcher’s visit. Patients were withdrawn from the study medication if any of the following were found; WBC less than 3000/mm3, absolute polymorphonuclear count less than 100000/mm3, acute or progressive decrease in hemoglobin or hematocrit, proteinuria less than 500 mg. for 24 hours, drug fever or significant rash.

The patients were queried at each secondary researcher’s visit regarding the dietary supplement or topical lotion they had used. A capsule count for the trial medication was done at each consultation to monitor compliance.

Biostatistical considerations
Each patient was classified as a treatment responder or nonresponder based on the following definition. Assessment measures were selected a priori, and criteria for clinical improvement and worsening were defined for each patient self-assessment and physician assessment (improvement category); joint pain/tenderness score and joint swelling score (improvement = decrease by 30%; worsening = increase by 30%). Treatment response was then defined as improvement in at least 2 of these 4 measures, one of which must be joint pain/tenderness or swelling, and ITO worsening any of the 4 measures. The study was designated with a 90% power for detecting a placebo response rate of 30% compared with a Cetyl Myristoleate and Cetyl Myristoleate plus GS, SC & HC response rate of 50%, assuming a 10% withdrawal rate. This resulted in a target sample size of 431 patients with an actual sample size of 382.

In short, the analytical method was the change in primary and secondary outcome measures from baselines to the last available follow-ups analyzed using t-tests for continuous data and chi-square tests for ordinal and categorical data. Mixed-model analyses were done to characterize the response patterns over time using SAS PROC MIXED for continuous data and a program named MIXOR for categorical and ordinal data. All other analyses were conducted using SAS version 6.08. All statistical tests were two-sided and P0.05 was the criterion for statistical significance.

Results Patient population
Four hundred thirty-one patients entered the study. Of these, 106 were randomized to receive Cetyl Myristoleate, 84 were randomized to receive Cetyl Myristoleate plus GS, SC & HC; 226 received a placebo. Fifteen psoriatics received Cetyl Myristoleate plus GS, SC & HC, plus CM-25% concentration topical at a 3X quantity ratio. Even though the study was sponsored by the owners of the respective private hospitals, recruitment was not limited to the typical fee-paying patients. Approximately 27% of the patients were actively recruited in the respective local area. Despite a prolonged accrual period and careful screening, the loss of approximately 11% of the starting participants occurred largely because of the inability to stop the use of tobacco and/or caffeinated beverages.

Fulfillment of final parameter of study size was accomplished by the substantial excess of volunteers wanting to enter the study - this coupled with the relatively short testing period required to validate the effects of Cetyl Myristoleate and Cetyl Myristoleate plus GS, SC & HC. Statistical Chart 1 outlines the baseline demographic, clinical, and laboratory variables. The duration of disease was 12 years. The Westergren ESR and CRP levels were mildly elevated. There were no statistically significant differences in any of these baseline parameters between the patients taking Cetyl Myristoleate and Cetyl Myristoleate plus GS, SC & HC and those taking placebo.

Compliance for both the Cetyl Myristoleate and Cetyl Myristoleate plus GS, SC & HC and placebo groups was quite high. There was a statistical trend toward those in the Cetyl Myristoleate and Cetyl Myristoleate plus GS, SC & HC group taking more tablets per day (96% compliance) than those in the placebo group (86% compliance) (P = 0.08). The probability of this observation was due to the rapid response of pain relief in the Cetyl Myristoleate groups.

Primary outcome measures
The Oversight Committee defined response based on a decision rule as outlined in Patients and Methods. Statistical Chart 1 shows that based on that definition of treatment response, using the last-visit analysis, response rates were 63.3% in the Cetyl Myristoleate group and 87.3% in the Cetyl Myristoleate plus GS, SC & HC group and 14.5% in the placebo group. Trends favoring Cetyl Myristoleate and Cetyl Myristoleate plus GS, SC & HC groups were noted in components of the response definition. Physician overall assessment showed an improvement of 58.1% for the patients using Cetyl Myristoleate alone and 84.2% for the patients using Cetyl Myristoleate plus GS, SC & HC. Patients experiencing worsening or no reaction totaled 1.0% in all groups, compared with improvement of 13.9% in placebo gradient overall assessment demonstrated 59.2% improvement in the Cetyl Myristoleate alone group and 88.2% in the Cetyl Myristoleate plus GS, SC & HC. Patients experiencing worsening or no reaction totaled 1.0% in all groups, compared with improvement of 16.1% in placebo group. Joint swelling scores improved in 47.2% in patients using Cetyl Myristoleate alone and 77.2% in patients using Cetyl Myristoleate plus GS, SC & HC. Patients experiencing worsening or no reaction totaled 1.0% in all groups, compared with improvement of 21.1% in placebo group.

Secondary and laboratory outcome measures
Analysis of secondary outcome results (Statistical Chart 2) demonstrated a significant reduction in the Spondylitis Articular Index in the Cetyl Myristoleate group and in the Cetyl Myristoleate plus GS, SC & HC-treated patients. Trends favoring the Cetyl Myristoleate group and in the Cetyl Myristoleate plus GS, SC & HC group were also seen in a reduced duration of early stiffness and in an improvement in the fingers-to-floor result. Laboratory outcome measures showed some statistically significant changes. Total neutrophils decreased in the Cetyl Myristoleate group and in the Cetyl Myristoleate plus GS, SC & HC group compared with the placebo group. The Westergren ESR significantly decreased in the Cetyl Myristoleate group and in the Cetyl Myristoleate plus GS, SC & HC groups compared with the placebo group. The CRP values were not significantly different and the values in ESR for the responders was not statistically significant from the nonresponders.

Withdrawals and adverse drug reactions
Statistical Chart 3 summarizes the data of patient exits from the study. Forty-nine patients withdrew from the study before completing the study, 16 from the Cetyl Myristoleate and 10 from Cetyl Myristoleate plus GS, SC & HC groups, 2 from the psoriatic group, and 21 from the placebo group. Follow-ups in all groups averaged approximately these measurements were combined, combined average - (mean ± SD 6.97 ± .2.64 months) (P=.06). Withdrawal of consent was the most common reason for discontinuing the study. Seven patients withdrew because of no improvement or worsening disease. Two patients had to be withdrawn from the study because of concurrent illnesses requiring conflicting medication. The majority of withdrawals, however, was the result of patient addictions to nicotine, caffeine and alcohol and the patient inability to cease these activities during the study period.

Statistical chart 2 displays the percentages of study patients showing improvement in the primary outcome variables (columns 1-3). The numbers to the right display the significance levels for the differences between treatment groups (columns 4-6). All of the significant levels are much less than 0.05, which means the differences between groups are considered statistically valid. For all four primary outcome variables (treatment response, physician assessment, patient assessment and joint swelling score), Cetyl Myristoleate & GS, SC, and HC did significantly better than the Cetyl Myristoleate group, and the Cetyl Myristoleate group did significantly better than placebo. The chart also displays the results for the secondary outcome variables. The averages (mean average as opposed to median or mode) are presented in columns 1-3 along with their standard deviations (statistical measurement of data variations). Again, the numbers to the right display the significance levels for the difference between treatment groups (columns 4-6). “NS,” means that there was no significant difference between any measured groups labeled as such. When the groups are significantly different from each other, the significance is displayed. None of the secondary outcome variables were significantly different between the Cetyl Myristoleate group and placebo. The Cetyl Myristoleate & GS, SC, HC, group did significantly better than the placebo for dwelling score, Enthesopathy index, spondylitis articular index and the modified Schober’s test. The Cetyl Myristoleate & GS, SC, HC, group did better than Cetyl Myristoleate alone for the joint pain/tenderness score and the modified Schober’s test.

The results of this trial suggest that Cetyl Myristoleate and Cetyl Myristoleate supporting formulas may be beneficial in the treatment of many forms of arthritic based diseases, including: psoriatic arthritis. The definition of response was determined a priori and included assessment of joint pain/tenderness and swelling as well as patient and physician overall assessments. Cetyl Myristoleate and supporting formulas produced the best treatment response by a factor of 72.8% more patients than did placebo. Considering the components of response individually Cetyl Myristoleate and supporting formulas resulted in 70.3% more patients having improved as assessed by physician, and 56.1% more having improved joint swelling. Therefore, while the amount of treatment response using Cetyl Myristoleate and Cetyl Myristoleate and supporting formulas seems to be consistent with the treatment affects on joint counts, it is obvious that there is a statistically significant improvement in the use of the CM with supporting formulas. The time-line based response rate of Cetyl Myristoleate and Cetyl Myristoleate supporting formulas, not adequately reflected in data, by patient, showed the majority of patients responding to Cetyl Myristoleate and Cetyl Myristoleate supporting formulas did so within the first three weeks. Also, not reflected in the data, was the continued use of Cetyl Myristoleate and Cetyl Myristoleate supporting formulas beyond the study time limits and dispensed on request to 21 patients. These 21 patients were determined to have received only marginal benefits from Cetyl Myristoleate and Cetyl Myristoleate supporting formulas but one more course of treatment showed responses approximately equal to the first patient response results.

Cetyl Myristoleate and Cetyl Myristoleate supporting formulas were well tolerated in this trial. This finding was not unexpected as Cetyl Myristoleate and the Cetyl Myristoleate supporting formula components are naturally occurring and have been used as diet supplementation for many years and are widely available singly and in various combinations. In summary, Cetyl Myristoleate and Cetyl Myristoleate supporting formulas appear to be beneficial in the treatment of a wide range of arthritic conditions including long standing and refractive cases.

Statistical Chart #1


Number of Patients 106 99 226
% Male 60 59 50
% Female 40 41 50
% White 32 83 38
% Black 15 9 12
% Hispanic 6 7 6
% Other 3 1 4

Statistical Chart #2
Primary % CM CM+ PLACEBO
Treatment Response 63.3 87.3 14.5
Doctor Overall Assessment 58.1 884.2 12.9
Patient Overall Assessment 39.2 88.2 16.1
Joint Swelling Score 47.5 77.2 21.1

Statistical Chart #3

Deceased Platelets 0 0 0
Increased Liver enzymes 0 0 0
Protocol Violations 7 3 4
Completed Study 90 87 205
Withdraw from Study 19 17 33
Worse 3 2 1
Adverse Material Reaction 0 0 1
Gastrointestinal Symptoms 3 2 3


1. Lightfoot, R.W., Jr.: Intermittent and periodic arthritic syndromes. Arthritic and Allied Conditions. 12Th edition. Edited by D.J. McCarty, W.J. Koopman Phdadelphia, Lea & Febiger, 1993.

2. Aho, K., Ahoven, P., Sievers, K., Tlilikanien, A.; Yersinia Arthritis and Related Diseases; Clinical and Immunogenetic Implications. Infection and Immunology in the rheumatic Diseases. Edited by D.C. Dumonde. Oxford, Blacksell Scientific Publications, 1976.

3. Diggie, P., Laing, Zeger, S., Analysis of Longitudinal Data. Oxford, Clarendon Press, 1994.

4. SAS Institute, Inc. SAS Technical Report P-229, SAS/STAT: Changes and Enhancements, Release 6.07., Cary, N.C., SAS Institute, Inc. 1992.

5. Hedecker, D., A random-effects ordinal regression model for multilevel analysis, Biometrics, 1994.

6. SAS Institute Inc: SAS/STAT User Guide: Version 6., 4th Edition., Cary, N.C., SAS Institute, 1990.

7. Toivanen, A.: Reactive Arthritis., Mosby Year Book. Edited by Klippel, J.H., Dieppe, D.A., Brooks, P., Carette, S., Dequek Keats. A.S., Kimberlly, R.Pl, Liang, M.H., Maini. R.N., A van de Putts, L.B., Sturrock, M.B., Urowitz. M.B., Wollheim. F.A., Zvaifler, M.J., London, Mosby-Year Book, 1994.

8. Fan, P.T., Yu, D Y,: Spondyloarthropathies. Textbook of Rheuth-matology., Vol. 1, 4th edition. Edited by, Kelley, W.N., Harris, E.D., Ruddy, S., Jr., Sledge, C.B., Philadelphia W.B. Saunders. 1993.

9. Smiley, J.D., Psoriatic arthritis., Bulletin of Rheumatic Disease, 44:, 1995.

10. Botanical Lipids. Effects in Inflammation. Immune Response, and Rheumatoid Arthritis. Rothman. D., et al. Seminars in Arthritis and Rheumatism, October 1995.

11. Anti-inflammatory Diet in Rheumatic Disease. Adam, O. European Journal of Clinical Nutrition, 1995.

12. Botanical Lipids. Effects in Inflammation, Immune Response, and Rheumatoid Arthritis. Rothman. D., et al. Seminars in Arthritis and Rheumatism, October 1995.

13. Cetyl Myristoleate Isolated from Swiss Albino Mice: An Apparent Protective Agent against Adjuvant Arthritis in Rata. Diehl, H., and May, E.L. Journal of Pharmaceutical Science, Vol. 83 March 1994.

14. Effects of Modulation of Inflammatory and Immune Parameters in Patients with Rheumatic and Inflammatory Disease Receiving Dietary Supplementation of N-3 and N-6 Fatty Acids. Kremer, j., Md, Lipids, 1996.

15. Glycosaminoglycan Supplements as Therapeutic Agents. Bucci , L., PhD., Nutritional Report, January 1996.

16. Rheumatoid Arthritis and Foods: A Patient Study, Borok, G., South African Family Pracice, October 1989.